S194/5.XXO.BU.1細(xì)胞

細(xì)胞類型： B**細(xì)胞

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

數(shù)量： 大量

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞形態(tài)： **樣

S194/5.XXO.BU.1細(xì)胞**類型： IgA (not secreted); kappa light chain (not secreted)

品系： BALB/c

ATCC Number： TIB-20?

相關(guān)**： 多發(fā)性骨髓瘤

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

Designations: S194/5.XXO.BU.1

Depositors: R Hyman, P Ralph, S Sarkar

Isotype: IgA (not secreted); kappa light chain (not secreted)

Biosafety Level: 1

S194/5.XXO.BU.1細(xì)胞Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus

Morphology: lymphoblast

Source: Disease: plasmacytoma; myeloma

Strain: BALB/c

Cell Type: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; not secreted

Permits/Forms: S194/5.XXO.BU.1細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Antigen Expression: H-2d; PC.1

Comments: Resistant to 0.1 mM 5-bromo-2'-deoxyuridine.

Derived from the S194/5.XXO-1 cell line (ATCC TIB-19).

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium, 90%; horse serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 22522: S194/5.XXO.BU.1細(xì)胞Hyman R, et al. Cell-specific antigens and immunoglobulin synthesis of murine myeloma cells and their variants. J. Natl. Cancer Inst. 48: 173-184, 1972. PubMed: 4119881