S1A.TB.4.8.2細(xì)胞

品系： BALB/c

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

數(shù)量： 大量

ATCC Number： TIB-27?

相關(guān)**： **瘤

細(xì)胞類型： T**細(xì)胞

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞形態(tài)： **樣

S1A.TB.4.8.2細(xì)胞Designations: S1A.TB.4.8.2 (replaces S1A.T4.G8)

Depositors: K Horibata, AW Harris

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus

Morphology: lymphoblast

Source: Disease: lymphoma

Strain: BALB/c

Cell Type: T lymphocyte;

Permits/Forms: S1A.TB.4.8.2細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: This line was derived from the S1A lymphoma.

The cells are resistant to 10 mM thymidine and 0.1 mM 5-bromo-2'-deoxyuridine.

Tested and found negative for ectromelia virus (mousepox).

Antigen Expression: Thy-1.2

Comments: This line was derived from the S1A lymphoma.

The cells are resistant to 10 mM thymidine and 0.1 mM 5-bromo-2'-deoxyuridine.

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: S1A.TB.4.8.2細(xì)胞Medium Renewal: Every 3 to 4 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 2 X 10 exp6 cells/ml.

Related Products: Recommended medium (without the additional serum described under ATCC Medium): ATCC 30-2002

Recommended serum: ATCC 30-2020

References: 22154: Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579

22529: Hyman R, Stallings V. Complementation patterns of Thy-1 variants and evidence that antigen loss variants "pre-exist" in the parental population. J. Natl. Cancer Inst. 52: 429-436, 1974. S1A.TB.4.8.2細(xì)胞PubMed: 4544550