S1A(Thy-1-b)細(xì)胞

運(yùn)輸方式： 凍存運(yùn)輸

ATCC Number： TIB-231?

相關(guān)**： **瘤

數(shù)量： 大量

細(xì)胞形態(tài)： **樣

細(xì)胞類型： T**細(xì)胞

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

生長狀態(tài)： 懸浮生長

S1A(Thy-1-b)細(xì)胞品系： BALB/c

Designations: S1A(Thy-1-b)

Depositors: R Hyman

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus

Morphology: lymphoblast

Source: Disease: lymphoma

Strain: BALB/c

Cell Type: S1A(Thy-1-b)細(xì)胞T lymphocyte;

Cellular Products: The cells secrete a mutant Thy-1 glycoprotein.

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Comments: This line is a Class B Thy-1 negative (Thy-1-) mutant derived from S1A.TB.4.8.2 (ATCC TIB-27).

The mutation affects the biosynthesis and/or addition of the glycophospholipid anchor.

The line may be useful in studies of the synthesis and assembly of glycophospholipid linked membrane proteins.

S1A(Thy-1-b)細(xì)胞The cells are resistant to 0.1 mM 5-bromo-2'-deoxyuridine.

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium, 90%; heat-inactivated horse serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 1807: Conzelmann A, et al. No glycolipid anchors are added to Thy-1 glycoprotein in Thy-1-negative mutant thymoma cells of four different complementation classes. Mol. Cell. Biol. 8: 674-678, 1988. S1A(Thy-1-b)細(xì)胞PubMed: 2895421