R2-10F6細(xì)胞

ATCC Number： CRL-2358?

是否是腫瘤細(xì)胞： 0

物種來源： 褐鼠

運(yùn)輸方式： 凍存運(yùn)輸

生長狀態(tài)： 懸浮生長

數(shù)量： 大量

**類型： IgG2b

Designations: R2-10F6 [R2-10F6.3C10.3F9]

Depositors: DH Presky

Isotype: IgG2b

R2-10F6細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma)

Morphology:

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against mouse interleukin 12 (IL-12) p40

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Comments: R2-10F6細(xì)胞The hybridoma cell line R2-10F6 [R2-10F6.3C10.3F9] was established by David Presky and Victoria Wilkinson in 1993 and subcloned twice.

This antibody is a useful tool for detection of mouse IL-12p40 by ELISA and Western Blot analysis.

R2-10F6 was formed by the fusion of SP2/0 mouse myeloma cells with splenocytes from an ***** female Lewis rat inoculated with recombinant mouse IL-12 p75.

Propagation: ATCC complete growth medium: Iscove's Modified Dulbecco's Medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 0.05 mM 2-mercaptoethanol and 100 U/ml recombinant human IL-6, 90%; fetal bovine serum, 10%.

R2-10F6細(xì)胞Temperature: 37.0°C

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Alternately, cultures can be established by centrifugation with subsequent resuspension in fresh culture medium.

Establish new cultures at 2 X 10 exp5 viable cells/ml and maintain at between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.

Preservation: Culture medium, 95%; DMSO, 5%

References: 34475: Wilkinson VL, et al. Characterization of anti-mouse IL-12 monoclonal antibodies and measurement of mouse IL-12 by ELISA. J. Immunol. Methods 189: 15-24, 1996. R2-10F6細(xì)胞PubMed: 8576576