UACC-3199細(xì)胞

組織來源： breast duct

器官來源： **

運(yùn)輸方式： 凍存運(yùn)輸

生長狀態(tài)： 貼壁生長

細(xì)胞形態(tài)： 上皮樣

ATCC Number： CRL-2983?

相關(guān)**： 其他**

年限： 58 years old

是否是腫瘤細(xì)胞： 0

物種來源： 人

UACC-3199細(xì)胞數(shù)量： 大量

Designations: UACC-3199

Depositors: K Brown

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial-like

Source: Organ: mammary gland; breast

Tissue: breast duct

Disease: infiltrating ductal carcinoma of the breast

Derived from metastatic site: axillary lymph node

Permits/Forms: UACC-3199細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: November 1994

Applications: UACC-3199 was derived from a 58 year-old female with infiltrating ductal carcinoma of the breast metastatic to the left axillary lymph nodes.

UACC-3199 cells are methylated at the BRAC 1 promoter site. This cell line is an excellent tool for research into aberrant cytosine methylation of CpG island sites and BRCA1 repression in sporadic breast cancer. It is also very useful as a methylated BRCA1 control.

Receptors: epidermal growth factor receptor (EGF), expressed

estrogen receptor, not expressed

progesterone receptor, not expressed

Tumorigenic: YES

Oncogene: c-erbB-2 positive

DNA Profile (STR): CSF1PO: 12

D13S317: 11

D16S539: 12

D5S818: 13

D7S820: 10

THO1: 9.3

UACC-3199細(xì)胞TPOX: 10, 13

vWA: 15, 17

Amelogenin: X

Age: 58 years old

Gender: female

Ethnicity: Caucasian

Comments: UACC-3199 was derived from a 58 year-old female with infiltrating ductal carcinoma of the breast metastatic to the left axillary lymph nodes. The patient had a family history of breast cancer.

UACC-3199 cells are methylated at the BRAC 1 promoter site. This cell line is an excellent tool for research into aberrant cytosine methylation of CpG island sites and BRCA1 repression in sporadic breast cancer. It is also very useful as a methylated BRCA1 control.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium:

fetal bovine serum to a final concentration of 5%

0.01 mg/ml transferrin (final conc.)

0.01 mg/ml insulin (final conc.)

5 ?g/ml (55 U/ml) catalase (final conc.)

3.6 ?g/ml (0.01 mM) hydrocortisone (final conc.)

extra 2 mM glutamine

Temperature: 37.0°C

Atmosphere: air, 100%

Growth Conditions: Cells grow very slowly. Cultures reach confluence in 2 to 3 weeks.

Growth Conditions: UACC-3199細(xì)胞Avoid inoculating vessels below recommended seeding density.

Subculturing: Protocol: Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Remove and discard culture medium.

Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 1.0 to 2.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37.0°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Transfer cell suspension to a centrifuge tube and spin at approximately 125 xg for 5 to 10 minutes. Discard supernatant.

Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 1 X 104 to 2 X 104 viable cells/cm2 is recommended.

Incubate cultures at 37.0°C. Subculture when the cell concentration is between 2 X 104 to 5 X 104 cells/cm2.

Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended.

Medium renewal: every 3 to 4 days

Preservation: Freeze medium: complete growth medium, 90%; DMSO, 10%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional serum described under ATCC Medium): ATCC 30-2008

Recommended serum: UACC-3199細(xì)胞ATCC 30-2020

0.25% (w/v) Trypsin - 0.53mM EDTA in Hank's BSS (w/o Ca++, Mg ++): ATCC 30-2101

Cell culture tested DMSO: ATCC 4-X

Phosphate-buffered saline: ATCC 30-2200

L-Glutamine solution, 200mM: ATCC 30-2214

References: 16173528: Rice JC, et al. Aberrant methylation of the BRCA1 CpG island promoter is associated with decreased BRCA1 mRNA in sporadic breast cancer cells. Oncogene 17(14): 1807-1812, 1998. PubMed: 9778046

16173529: Rice JC, et al. Transcriptional repression of BRCA1 by aberrant cytosine methylation, histone hypoacetylation and chromatin condensation of the BRCA1 promoter. Nucleic Acids Res. 28(17): 3233-3239, 2000. PubMed: 10954590