R1E/TL8x.1細胞
數(shù)量： 大量
運輸方式： 凍存運輸
細胞類型： T**細胞
是否是腫瘤細胞： 0
物種來源： 小鼠
ATCC Number： TIB-43?
器官來源： 胸腺
相關**： **瘤
R1E/TL8x.1細胞細胞形態(tài)： **樣
品系： C58/J
生長狀態(tài)： 懸浮生長
Designations: R1E/TL8x.1
Depositors: R Hyman
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus
Morphology: lymphoblast
Source: R1E/TL8x.1細胞Organ: thymus
Strain: C58/J
Disease: lymphoma
Cell Type: T lymphocyte;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression: H-2k; Thy-1.2
Comments: Unlike the parental line R1.1, these cells do not express TL antigen.
Tested and found negative for ectromelia virus (mousepox).
Propagation: R1E/TL8x.1細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: culture medium, 92.5%; DMSO, 7.5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2040
parental cell line:R1E/TL8x.1細胞ATCC TIB-42
Cell culture tested DMSO:ATCC 4-X
References: 22247: Hyman R, Stallings V. Characterization of a TL- variant of a homozygous TL+ mouse lymphoma. Immunogenetics 3: 75-84, 1976.
