R17 217.1.3細胞
生長狀態(tài)： 懸浮生長
運輸方式： 凍存運輸
數(shù)量： 大量
是否是腫瘤細胞： 0
物種來源： 大鼠
ATCC Number： TIB-219?
細胞形態(tài)： **樣
**類型： IgG2a , IgG2a
R17 217.1.3細胞Designations: R17 217.1.3
Depositors: J Lesley
Isotype: IgG2a , IgG2a
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension, suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: R17 217.1.3細胞immunoglobulin; monoclonal antibody; against mouse transferrin receptor
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Comments: Animals were immunized with the mouse erythroleukemia line 745.6.
Spleen cells were fused with S194/5.XXO.BU.1 myeloma cells.
This antibody stains brighter by immunofluorescence than does R17 208.2 (ATCC TIB-220).
The antibody does not prevent cell proliferation.
Tested and found negative for ectromelia virus (mousepox).
Propagation: R17 217.1.3細胞ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.02 mM 2-mercaptoethanol and 10% horse serum
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 4 X 10 exp4 cells/ml and do not allow the cell concentration to exceed 4 X 10 exp5 cells/ml.
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2040
References: 58016: R17 217.1.3細胞Lesley J, et al. Expression of transferrin receptor on murine hematopoietic progenitors. Cell. Immunol. 83: 14-25, 1984. PubMed: 6319033
