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產(chǎn)品資料

2D4細(xì)胞

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產(chǎn)品名稱: 2D4細(xì)胞
產(chǎn)品型號(hào): 2D4
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無相關(guān)文檔

簡(jiǎn)單介紹

2D4細(xì)胞應(yīng)如何避免細(xì)胞污染,細(xì)胞污染的種類可分成**、酵母菌、霉菌、病毒和霉?jié){菌。主要的污染原因?yàn)闊o菌操作技術(shù)不當(dāng)、操作室環(huán)境不佳、污染之血清和污染之細(xì)胞等。嚴(yán)格之無菌操作技術(shù)、清潔的環(huán)境、與品質(zhì)良好之細(xì)胞來源和培養(yǎng)基配制是減低污染之*好方法。2D4細(xì)胞何時(shí)須更換培養(yǎng)基?視細(xì)胞生長(zhǎng)密度而定,或遵照細(xì)胞株基本數(shù)據(jù)上之更換時(shí)間,按時(shí)更換培養(yǎng)基即可。


2D4細(xì)胞  的詳細(xì)介紹

2D4細(xì)胞

生長(zhǎng)狀態(tài): 懸浮生長(zhǎng)

ATCC Number: TIB-185?

細(xì)胞形態(tài): **樣

**類型: IgM

運(yùn)輸方式: 凍存運(yùn)輸

數(shù)量: 大量

是否是腫瘤細(xì)胞: 0

物種來源: 小鼠

Designations: 2D4

2D4細(xì)胞Depositors: WW Young

Isotype: IgM

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against asialo GM2

Permits/Forms: 2D4細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with asialo GM2 non-covalently adsorbed to naked Salmonella minnesota.

Spleen cells were fused with NS-1 myeloma cells.

The antibody reacts with a distinct steric portion of the carbohydrate moiety of asialo GM2, specifically the C6 hydroxyl bearing group of the terminal N-acetylgalactosamine residue.

Tested and found negative for ectromelia virus (mousepox).

Propagation: 2D4細(xì)胞ATCC complete growth medium: RPMI 1640 medium, 85%; fetal bovine serum, 15%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.

References: 971: Young WW Jr., et al. Production of monoclonal antibodies specific for two distinct steric portions of the glycolipid ganglio-N-triosylceramide (Asialo GM2). J. Exp. Med. 150: 1008-1019, 1979. PubMed: 512581

22944: Young WW J, Hakomori SI. Therapy of mouse lymphoma with monoclonal antibodies to glycolipid: selection of low antigenic variants in vivo. Science 211: 487-489, 1981. 2D4細(xì)胞PubMed: 7455688

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