2D12細(xì)胞
數(shù)量： 大量
生長狀態(tài)： 懸浮生長
細(xì)胞形態(tài)： **樣
**類型： IgG2a; kappa light chain
ATCC Number： CRL-1689?
運輸方式： 凍存運輸
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
Designations: 2D12
2D12細(xì)胞Depositors: JJ Schlesinger
Isotype: IgG2a; kappa light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:2D12細(xì)胞 immunoglobulin; monoclonal antibody; against yellow fever virus (vaccine strains and Asibi strain)
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Antigen Expression: H-2d
Comments: Mice were immunized with the 17D strain of yellow fever virus.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody does not cross-react with other flaviviruses.
2D12細(xì)胞Antibody reactivity can be assayed by hemagglutination inhibition, complement fixation and immunofluorescence.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: RPMI 1640 medium containing non-essential amino acids, 20 mM HEPES, 1 mM sodium pyruvate, 2 mM L-glutamine, and 0.02 mM 2-mercaptoethanol, 85%; fetal bovine serum, 15%
Subculturing: Medium Renewal: Every 3 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Maintain cell concentration between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%
References: 1014: Schlesinger JJ, et al. 2D12細(xì)胞Monoclonal antibodies distinguish between wild and vaccine strains of yellow fever virus by neutralization, hemagglutination inhibition and immune precipitation of the virus envelope protein. Virology 125: 8-17, 1983. PubMed: 6187129
1015: Monath TP, et al. Yellow fever monoclonal antibodies: type-specific and cross-reactive determinants identified by immunofluorescence. Am. J. Trop. Med. Hyg. 33: 695-698, 1984. PubMed: 6206738
