GS-109-V-34細胞
生長狀態(tài)： 貼壁生長
ATCC Number： CRL-1613?
相關**： 加德納綜合征
細胞形態(tài)： 成纖維樣
運輸方式： 凍存運輸
數(shù)量： 大量
器官來源： 皮膚
年限： 6 years
是否是腫瘤細胞： 0
物種來源： 人
GS-109-V-34細胞Designations: GS-109-V-34
Depositors: EJ Gardner
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: skin
Disease: Gardner's syndrome
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Tumorigenic: No
Cytogenetic Analysis: This cell line has the normal female karyotype, 46,XX. The modal chromosome number was 46, occurring in 84% of cells, and the rate of polyploidy was 2.8%.The X chromosome was paired. There were no consistent aberrations in all analyzed cells.
Age: 6 years
Gender: female
Ethnicity: Caucasian
Comments: The tissue donor suffered from Gardner's syndrome.
The cells exist in an initiated state as opposed to a normal or a transformed state.
Propagation: GS-109-V-34細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Temperature: 37.0°C
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
GS-109-V-34細胞Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium GS-109-V-34細胞(without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
