GS-109-V-63 [G96]細(xì)胞

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

器官來(lái)源： 皮膚

ATCC Number： CRL-1614?

相關(guān)**： 加德納綜合征

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 人

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

年限： 13 years

GS-109-V-63 [G96]細(xì)胞細(xì)胞形態(tài)： 成纖維樣

Designations: GS-109-V-63 [G96]

Depositors: EJ Gardner

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Organ: skin

Disease: Gardner's syndrome

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. GS-109-V-63 [G96]細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: No

Cytogenetic Analysis: This is a normal diploid human cell line with 46,XY karyotype. The modal chromosome number was 46, occurring in 60% of cells. The rate of polyploidy was 2.4%. Both X and Y chromosomes were normal.

Age: 13 years

Gender: male

Ethnicity: Caucasian

Comments: The tissue donor suffered from Gardner's syndrome.

The cells exist in an initiated state as opposed to a normal or a transformed state.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.

Temperature: 37.0°C

Subculturing: Protocol:

GS-109-V-63 [G96]細(xì)胞Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor pahse

Related Products: Recommended medium GS-109-V-63 [G96]細(xì)胞(without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020