2E.6細(xì)胞

是否是腫瘤細(xì)胞： 0

物種來源： 大鼠

數(shù)量： 大量

細(xì)胞形態(tài)： **樣

**類型： IgG1

運(yùn)輸方式： 凍存運(yùn)輸

ATCC Number： HB-128?

生長狀態(tài)： 懸浮生長

Designations: 2E.6

Depositors: JM Davie

2E.6細(xì)胞Isotype: IgG1

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse 2E.6細(xì)胞(myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against mouse IgG3

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Comments: 2E.6細(xì)胞Animals were immunized with mouse myeloma proteins of the IgG1 (MOPC-21), IgG2a (HOPC-1), IgG2b (MPC-11), IgG3 (J606 and GAC-39) and IgM (M104E) subclasses.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with non-essential amino acids, 85%; horse serum, 10%; fetal bovine serum, 5%. If desired, the fetal bovine serum may be omitted

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. 2E.6細(xì)胞Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.