2C1H7 [(PTA-2846)]細胞

ATCC Number： CRL-2746?

品系： BALB/c

運輸方式： 凍存運輸

生長狀態(tài)： 懸浮生長

器官來源： 脾

數量： 大量

是否是腫瘤細胞： 0

物種來源： 小鼠

細胞形態(tài)： **樣

2C1H7 [(PTA-2846)]細胞**類型： IgG1 kappa , IgG1; kappa light chain

Designations: 2C1H7 [(PTA-2846)]

Depositors: Tularik, Inc.

Isotype: IgG1 kappa , IgG1; kappa light chain

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension, suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Organ: spleen

Strain: BALB/c

Cell Type: 2C1H7 [(PTA-2846)]細胞hybridoma: B lymphocyte;

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Comments: Animals were immunized with a peptide derived from human beta tubulin with the sequence [H]-KATMSGVTTCLRFPGQLNA-[OH]. [57814]

Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. [57814]

The antibody recognizes modified beta tubulin (an isotype of beta tubulin). It is modified at a cysteine residue at amino acid position 239. [57814]

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose supplemented with 0.1 mM non-essential amino acids, 80%; fetal bovine serum, 20%

Temperature: 37.0°C

Subculturing: Protocol: 2C1H7 [(PTA-2846)]細胞Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/ml. Maintain cultures at a cell concentration between 2 x 105 and 5 x 105 cells/ml.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: parental cell line:ATCC PTA-2686

References: 57814: 2C1H7 [(PTA-2846)]細胞Beckmann H, Edit S. Detection method for monitoring .beta. tubulin isotype specific modification. US Patent 6,306,615 dated Oct 23 2001