GS-109-V-21細(xì)胞
器官來源： 皮膚
細(xì)胞類型： 成纖維細(xì)胞
是否是腫瘤細(xì)胞： 0
物種來源： 人
運(yùn)輸方式： 凍存運(yùn)輸
生長狀態(tài)： 貼壁生長
ATCC Number： CRL-1643?
相關(guān)**： 加德納綜合征
年限： 17 years
數(shù)量： 大量
GS-109-V-21細(xì)胞細(xì)胞形態(tài)： 成纖維樣
Designations: GS-109-V-21
Depositors: EJ Gardner
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: skin
Disease: Gardner's syndrome
Cell Type: fibroblast
Permits/Forms: GS-109-V-21細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Cytogenetic Analysis: This is a pseudodiploid human cell line with the karyotype, 46,XX,-2,2q+. The modal chromosome number was 46, occurring in 40% of cells. The rate of polyploidy was 4%. The marker, 2q+, was present in all metaphases. There was only one copy of normal N2. The X chromosome was paired.
Age: 17 years
Gender: female
Ethnicity: Caucasian
Comments: The tissue donor suffered from Gardner's syndrome.
The cells exist in an initiated state as opposed to a normal or a transformed state.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing:GS-109-V-21細(xì)胞 Protocol: Re
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: GS-109-V-21細(xì)胞Twice per week
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2007
recommended serum:ATCC 30-2020
