GS-109-IV-8細(xì)胞

器官來(lái)源： 皮膚

細(xì)胞形態(tài)： 成纖維樣

運(yùn)輸方式： 凍存運(yùn)輸

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 人

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

年限： 48 years

ATCC Number： CRL-1672?

相關(guān)**： 加德納綜合征

數(shù)量： 大量

GS-109-IV-8細(xì)胞Designations: GS-109-IV-8

Depositors: EJ Gardner

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Organ: skin

Disease: Gardner's syndrome

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. GS-109-IV-8細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Age: 48 years

Gender: female

Ethnicity: Caucasian

Comments: The tissue donor suffered from Gardner's syndrome.

The cells are said to exist in an initiated state as opposed to a normal or transformed state.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. GS-109-IV-8細(xì)胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended

Medium Renewal: 2 to 3 times per week

Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes). GS-109-IV-8細(xì)胞Add fresh medium, aspirate and dispense into new flasks.