Y13-259細(xì)胞
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
數(shù)量： 大量
運(yùn)輸方式： 凍存運(yùn)輸
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 褐鼠
ATCC Number： CRL-1742?
細(xì)胞形態(tài)： **樣
**類型： IgG1
Designations: Y13-259
Y13-259細(xì)胞Depositors: JR Stephenson, ME Furth
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus (B cell); Rattus norvegicus (myeloma) deposited as rat (B cell); rat (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against the p21 transforming protein encoded by the v-ras and c-ras (ras) oncogenes
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Y13-259細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with syngeneic normal rat kidney cells (NRK) that had been infected with Harvey murine sarcoma virus.
Spleen cells were fused with 210RCY3 Ag 1.2.3 myeloma cells.
The antibody reacts with both the Harvey (Ha-ras) and Kirsten (Ki-ras) p21 proteins.
The antibody also precipitates an endogenous p21 from a variety of rodent, carnivore and primate cell lines.
Propagation: Y13-259細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1320: Furth ME, et al. Monoclonal antibodies to the p21 products of the transforming gene of Harvey murine sarcoma virus and of the cellular ras gene family. J. Virol. 43: 294-304, 1982. Y13-259細(xì)胞PubMed: 6287003
