2D10細(xì)胞

細(xì)胞形態(tài)： **樣

**類型： IgG2b

年限： *****

ATCC Number： CRL-2226?

品系： Lewis

生長狀態(tài)： 懸浮生長

數(shù)量： 大量

運(yùn)輸方式： 凍存運(yùn)輸

器官來源： 其他

是否是腫瘤細(xì)胞： 0

2D10細(xì)胞物種來源： 大鼠

Designations: 2D10

Depositors: SE Buchholz

Isotype: IgG2b

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Organ: lymph node, popliteal

Strain: Lewis

Cell Type: hybridoma: B lymphocyte;

Cellular Products: 2D10細(xì)胞immunoglobulin; monoclonal antibody; against mouse B7.2

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Age: *****

Comments: Animals were immunized in the hind footpad with irradiated 5C2 mouse B cell lymphoma cells that had been cultured in the presence of dibutyryl-cAMP.

Popliteal lymph node cells were fused with Sp2/0-Ag14 myeloma cells.

The antibody will neutralize the B7.2 antigen, but does not react with the B7.1 antigen.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium:

0.05 mM 2-mercaptoethanol

fetal bovine serum to a final concentration of 10%

Temperature: 37.0°C

Subculturing: 2D10細(xì)胞Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 0.5 X 10 exp5 to 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: Culture medium, 95%; DMSO, 5%

References: 22286: Powers GD, et al. Expression and functional analysis of murine B7 delineated by a novel monoclonal antibody. Cell. Immunol. 153: 298-311, 1994. PubMed: 7509723

22935: Nabavi N, et al. Signalling through the MHC class II cytoplasmic domain is required for antigen presentation and induces B7 expression. Nature 360: 266-268, 1992. PubMed: 1279442

23485: Chen C, et al. Monoclonal antibody 2D10 recognizes a novel T cell costimulatory molecule on activated murine B lymphocytes. J. Immunol. 152: 2105-2114, 1994. 2D10細(xì)胞PubMed: 7510738