Y13-238細胞

細胞形態(tài)： **樣

**類型： IgG2a

ATCC Number： CRL-1741?

運輸方式： 凍存運輸

數(shù)量： 大量

是否是腫瘤細胞： 0

物種來源： 褐鼠

生長狀態(tài)： 混合型生長

Designations: Y13-238

Depositors: JR Stephenson, ME Furth

Isotype: IgG2a

Y13-238細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: mixed, adherent and suspension

Organism: Rattus norvegicus (B cell); Rattus norvegicus (myeloma) deposited as rat (B cell); rat (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against the p21 transforming protein encoded by the v-ras and c-ras (ras) oncogenes

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Comments: Rats were immunized with syngeneic normal rat kidney cells (NRK) that had been infected with Harvey murine sarcoma virus.

Spleen cells were fused with 210RCY3 Ag 1.2.3 myeloma cells.

The antibody is specific for the Ha-ras (Harvey) p21 and does not react with the p21 of Ki-ras (Kirsten).

Propagation: Y13-238細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Growth Conditions: Maintain the cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml. Do not allow the density to exceed 1 X 10(6) cells/ml.

Y13-238細胞Subculturing: Protocol: Dislodge attached cells from the substrate with a cell scraper, aspirate and dispense into new flasks.

Medium Renewal: two to three times weekly

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 1320: Furth ME, et al. Monoclonal antibodies to the p21 products of the transforming gene of Harvey murine sarcoma virus and of the cellular ras gene family. J. Virol. 43: 294-304, 1982.Y13-238細胞 PubMed: 6287003