Y-3細(xì)胞

細(xì)胞形態(tài)： **樣

**類型： IgG2b; kappa light chain

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

ATCC Number： HB-176?

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

數(shù)量： 大量

運(yùn)輸方式： 凍存運(yùn)輸

Designations: Y-3

Y-3細(xì)胞Depositors: C Janeway

Isotype: IgG2b; kappa light chain

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against H-2 K b

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Comments: Animals were immunized with Con A stimulated spleen cells from BALB.B mice.

Spleen cells were fused with Sp2/0 myeloma cells.

The antibody cross-reacts with the k, p, q, r and s H-2 haplotypes.

Tested and found negative for ectromelia virus (mousepox).

Y-3細(xì)胞Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 1308: Hammerling GJ, et al. Localization of allodeterminants on H-2Kb antigens determined with monoclonal antibodies and H-2 mutant mice. Proc. Natl. Acad. Sci. USA 79: 4737-4741, 1982. Y-3細(xì)胞PubMed: 6181513