Y-3P細胞
運輸方式： 凍存運輸
是否是腫瘤細胞： 0
物種來源： 小鼠
數(shù)量： 大量
生長狀態(tài)： 懸浮生長
ATCC Number： HB-183?
細胞形態(tài)： **樣
**類型： IgG2a
Designations: Y-3P
Depositors: C Janeway
Y-3P細胞Isotype: IgG2a
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:Y-3P細胞 immunoglobulin; monoclonal antibody; against I-A; cytotoxic
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Comments: Animals were immunized with spleen cells from (BALB/c X C57BL/6)F1 mice and boosted with spleen cells from BALB/c mice immunized with either BALB.B or C57BL/6 T cell blasts.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody reacts with I-A b, f, p, q, r, s, u, and v haplotypes and weakly with I-A k.
Y-3P細胞It cross-reacts with all rat I-A like molecules.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1312: Janeway CA Jr., et al. Monoclonal antibodies specific for Ia glycoproteins raised by immunization with activated T cells: possible role of T cellbound Ia antigens as targets of immunoregulatory T cells. J. Immunol. 132: 662-667, 1984.Y-3P細胞 PubMed: 6228596
