TSHR-T3-365細(xì)胞

生長狀態(tài)： 懸浮生長

數(shù)量： 大量

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

運輸方式： 凍存運輸

細(xì)胞形態(tài)： **樣

**類型： IgG2a; kappa light chain

ATCC Number： CRL-2684?

Designations: TSHR-T3-365

TSHR-T3-365細(xì)胞Depositors: H Loosfelt, E Milgrom

Isotype: IgG2a; kappa light chain

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against human thyroid stimulating hormone (TSH) receptor beta subunit, intracellular domain

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Tumorigenic: Yes

Comments: Animals were immunized with recombinant human thyroid stimulating hormone (TSH) receptor expressed in. E. coli.

Spleen cells were fused with Sp2/0 mouse myeloma cells.

The antibody recognizes the C-terminal intracellular domain (amino acids domain 604-764), beta subunit of the human thyroid stimulating hormone (TSH) receptor. It recognizes an intracellular epitope of the beta subunit anchored into the cellular membrane.

The TSHR-R5T-34 (ATCC CRL-2683) antibody recognizes a N-terminal extracellular epitope of the beta subunit while the TSHR-T3-365 (ATCC CRL-2684) antibody recognizes a C-terminal intracellular subunit of the beta subunit.

The antibodies can be used in Western Blot, ELISA, immunocytochemistry, and immunohistochemistry assays of the human TSH receptor.

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90%; heat-inactivated horse serum, 10%

Temperature: 37.0°C

Subculturing: TSHR-T3-365細(xì)胞Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.

Maintain cell density between 2 X 10 exp5 and 1 X 10 exp6 viable cells/ml.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

References: 56150: Loosfelt H, et al. TSHR-T3-365細(xì)胞Two-subunit structure of the human thyrotropin receptor. Proc. Natl. Acad. Sci. USA 89: 3765-3769, 1992. PubMed: 1570295