2H6-C2細(xì)胞

ATCC Number： CRL-1853?

數(shù)量： 大量

細(xì)胞形態(tài)： **樣

**類型： IgG2b

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

運(yùn)輸方式： 凍存運(yùn)輸

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

Designations: 2H6-C2

2H6-C2細(xì)胞Depositors: CA Whetstone

Isotype: IgG2b

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

2H6-C2細(xì)胞Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against Bovine herpesvirus 1 (BHV-1, also known as infectious bovine rhinotracheitis virus (IBRV))

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Tumorigenic: Yes

Comments: Animals were immunized with BHV-1.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

2H6-C2細(xì)胞The antibody is specific for the gD glycoprotein of BHV-1 and is used by the National Veterinary Services Laboratories of the USDA for in vitro potency testing of adjuvantated, inactivated BHV-1 vaccines.

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 92.5%; horse serum, 5%; fetal bovine serum, 2.5%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 24382: . . Conf. Res. 2H6-C2細(xì)胞Workers Anim. Dis. : 54, 1987.