GR-20細(xì)胞

運(yùn)輸方式： 凍存運(yùn)輸

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 大鼠

數(shù)量： 大量

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

細(xì)胞形態(tài)： **樣

**類(lèi)型： IgG2a; kappa light chain

ATCC Number： CRL-2024?

Designations: GR-20

GR-20細(xì)胞Depositors: SW Russell

Isotype: IgG2a; kappa light chain

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against mouse receptor for gamma interferon

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. GR-20細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with mouse gamma interferon receptor protein purified from solubilized WEHI-3 cells (see ATCC TIB-68).

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

The antibody reacts with an extracellular epitope on the binding site of the mouse receptor for gamma interferon with an avidity of 3.33 X 10 exp-3 l/mol.

It blocks both interferon - receptor binding, and induction of macrophage mediated tumor cell killing.

It does not have agonist activity in the induction of macrophage killing.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. GR-20細(xì)胞To make the complete growth medium, add the following components to the base medium:

0.05 mM 2-mercaptoethanol

fetal bovine serum to a final concentration of 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 22141: Basu M, et al. A monoclonal antibody against the ligand binding site of the receptor for mouse interferon-gamma. J. Interferon Res. 9: 551-562, 1989. PubMed: 2477471

22295: Basu M, et al. Purification and partial characterization of a receptor protein for mouse interferon gamma. Proc. Natl. Acad. Sci. USA 85: 6282-6286, 1988. PubMed: 2970637

22785: LeClaire RD, et al. Characterization and use of monoclonal and polyclonal antibodies against the mouse interferon-gamma receptor. J. Leukocyte Biol. 51: 507-516, 1992. GR-20細(xì)胞PubMed: 1376354