2T8-3E10細(xì)胞

ATCC Number： HB-8213?

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

運(yùn)輸方式： 凍存運(yùn)輸

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

數(shù)量： 大量

細(xì)胞形態(tài)： **樣

**類(lèi)型： IgM

Designations: 2T8-3E10

2T8-3E10細(xì)胞Depositors: Dana Farber Cancer Inst.

Isotype: IgM

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: 2T8-3E10細(xì)胞immunoglobulin; monoclonal antibody; against human T cell antigen (Ti1b antigen) expressed only on the CT8III cytotoxic T lymphocyte clone

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Tumorigenic: Yes

Comments: Animals were immunized with CT8III cells.

Spleen cells were fused with NS-1 myeloma cells.

The antibody does not react with human thymocytes or resting or activated peripheral T cells.

Propagation: 2T8-3E10細(xì)胞ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 64%; NCTC 109 medium, 10%; L-glutamine, 2%; sodium pyruvate, 1%; hypoxanthine-thymidine, 1%; oxaloacetic acid and insulin, 1%; horse serum, 10%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 3546: Schlossman SF, et al. 2T8-3E10細(xì)胞Monoclonal antibodies that recognize human T cells. US Patent 4,550,086 dated Oct 29 1985