2F-2B細(xì)胞

品系： C3H/HeJ

ATCC Number： CRL-2168?

數(shù)量： 大量

組織來源： vascular epithelium

細(xì)胞形態(tài)： 上皮樣

細(xì)胞類型： SV40轉(zhuǎn)化細(xì)胞

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

生長狀態(tài)： 貼壁生長

器官來源： 腋窩**結(jié)

運(yùn)輸方式： 凍存運(yùn)輸

2F-2B細(xì)胞年限： *****

Designations: 2F-2B

Depositors: KA O'Connell

Biosafety Level: 2 [CELLS CONTAIN PAPOVAVIRUS ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus

Morphology: epithelial

Source: Organ: axillary lymph node

Strain: C3H/HeJ

Tissue: vascular epithelium

Cell Type: 2F-2B細(xì)胞endothelialSV40 transformed

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Tumorigenic: Yes

Antigen Expression: H-2 K; VCAM

Age: *****

Gender: male

Comments: The 2F-2B cell line was derived from a nodular cutaneous solid tumor in nude mice injected with SVEC4-10EE2 (see ATCC CRL- 2167). The line was cloned in 1991 by limiting dilution. The cells are sensitive in vitro to both macrophages and to natural killer (NK) cells.They express the cell surface major histocompatibility complex class I antigen, H-2 k, of the parental cell line, and vascular cell adhesion molecule (VCAM).The cells stain positively for SV40 T antigen.

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; heat-inactivated fetal bovine serum, 10%

2F-2B細(xì)胞Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended

Medium Renewal: Twice per week

Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin.

Add fresh trypsin solution (1 to 2 ml), and let the culture sit at room temperature until the cells detach.

Add fresh medium, aspirate and dispense into new flasks.

Preservation: Culture medium, 95%; DMSO, 5%

References: 22840: O'Connell KA, Edidin M. A mouse lymphoid endothelial cell line immortalized by simian virus 40 binds lymphocytes and retains functional characteristics of normal endothelial cells. J. Immunol. 144: 521-525, 1990. PubMed: 2153170

23172: O'Connell KA, Rudmann AA. Cloned spindle and epithelioid cells from murine Kaposi's sarcoma-like tumors are of endothelial origin. J. Invest. Dermatol. 100: 742-745, 1993. PubMed: 8496612

23187: O'Connell K, et al. 2F-2B細(xì)胞Endothelial cells transformed by SV40 T antigen cause Kaposi's sarcomalike tumors in nude mice. Am. J. Pathol. 139: 743-749, 1991. PubMed: 1928299