2G8.D6細胞

ATCC Number： HB-8190?

數(shù)量： 大量

是否是腫瘤細胞： 0

物種來源： 小鼠

運輸方式： 凍存運輸

生長狀態(tài)： 懸浮生長

細胞形態(tài)： **樣

**類型： IgG1; kappa light chain

Designations: 2G8.D6

2G8.D6細胞Depositors: Massachusetts Institute of Technology

Isotype: IgG1; kappa light chain

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products:2G8.D6細胞 immunoglobulin; monoclonal antibody; against o-phosphotyrosine and proteins which contain phosphotyrosine (tyrosine phosphate)

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Tumorigenic: Yes

Comments: Animals were immunized with p-azobenzylphosphonate covalently linked to a carrier protein.

2G8.D6細胞Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10%

Temperature: 37.0°C

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 3545: Eisen HN, et al. 2G8.D6細胞Production and use of monoclonal antibodies to phosphotyrosine-containing proteins. US Patent 4,543,439 dated Sep 24 1985