GK-5細(xì)胞
細(xì)胞形態(tài)： **樣
細(xì)胞類型： B**細(xì)胞
數(shù)量： 大量
是否是腫瘤細(xì)胞： 0
物種來源： 人
ATCC Number： CRL-1834?
運(yùn)輸方式： 凍存運(yùn)輸
器官來源： 外周血
GK-5細(xì)胞生長狀態(tài)： 懸浮生長
Designations: GK-5
Depositors: JF Kearney
Biosafety Level: 2 [Cells Contain HERPESVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Organ: peripheral blood
Cell Type: GK-5細(xì)胞B lymphoblast; Epstein-Barr virus (EBV) transformed
Cellular Products: immunoglobulin; monoclonal antibody; against human kappa light chain
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: GK-5 is a human lymphoblastoid cell line derived from the GM1500-GTG-A12 EBV transformed cell line.
The cells secrete very low levels (less than 10 ng/ml) of human kappa light chain.
The line has been used successfully to prepare human hybridomas.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. GK-5細(xì)胞To make the complete growth medium, add the following components to the base medium:
0.05 mM 2-mercaptoethanol
fetal bovine serum to a final concentration of 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 21879: Kearney JF. Hybridomas and monoclonal antibodies In: Kearney JF. Fundamental immunology: New YorkRaven Press pp. 751-766, 1984
22889: Satoh J, et al. GK-5細(xì)胞Human monoclonal autoantibodies that react with multiple endocrine organs. N. Engl. J. Med. 309: 217-220, 1983. PubMed: 6866036
