Lu Vin細(xì)胞
年限: 6 years
器官來源: 皮膚
生長狀態(tài): 貼壁生長
運輸方式: 凍存運輸
細(xì)胞形態(tài): 成纖維樣
細(xì)胞類型: 成纖維細(xì)胞
是否是腫瘤細(xì)胞: 0
物種來源: 人
數(shù)量: 大量
ATCC Number: CRL-1144?
相關(guān)**: Ehlers-Danlos綜合征
Lu Vin細(xì)胞Designations: Lu Vin
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: skin
Disease: Ehlers-Danlos syndrome, type I (autosomal dominant type)
Cell Type: fibroblast
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Lu Vin細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 6 years
Gender: female
Comments: The tissue donor suffered from Ehlers-Danlos syndrome (autosomal dominant type), and was the daughter of Mar Vin (ATCC CRL-1138) and the niece of Ron Har (ATCC CRL-1131).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. Lu Vin細(xì)胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove spent medium, add fresh 0.25% trypsin for 1 to 2 minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Lu Vin細(xì)胞Subculture every 7 to 9 days.