TR 310細(xì)胞

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

ATCC Number： HB-219?

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

是否是腫瘤細(xì)胞： 0

物種來源： 大鼠

細(xì)胞形態(tài)： **樣

**類型： IgG2b

Designations: TR 310

TR 310細(xì)胞Depositors: IL Weissman

Isotype: IgG2b

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: TR 310細(xì)胞immunoglobulin; monoclonal antibody; against mouse T cell receptor

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Comments: Animals were immunized with the Tk1 murine T cell lymphoma.

Spleen cells were fused with P3X63Ag8.653 myeloma cells.

TR 310細(xì)胞The antibody reacts with a deteminant on the mouse T cell antigen receptor.

It recognizes a determinant encoded by the Vb7 (V beta 7) gene segment.

Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine, 1 mM sodium pyruvate and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. TR 310細(xì)胞Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.