Lu Rob細(xì)胞

ATCC Number： CRL-1397?

相關(guān)**： 其他**

細(xì)胞形態(tài)： 成纖維樣

年限： 50 years

器官來(lái)源： 皮膚

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 人

運(yùn)輸方式： 凍存運(yùn)輸

Lu Rob細(xì)胞數(shù)量： 大量

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

Designations: Lu Rob

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Organ: skin

Disease: Lu Rob細(xì)胞Ehlers-Danlos syndrome, type IV

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Age: 50 years

Gender: female

Comments: The tissue donor suffered from type IV Ehlers-Danlos syndrome.

Propagation: Lu Rob細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Remove spent medium, add fresh 0.25% trypsin for 3 to 5 minutes, remove trypsin and let the culture sit at 37C for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Lu Rob細(xì)胞Subculture every 6 to 8 days.