TRA-171細胞

生長狀態(tài)： 貼壁生長

年限： larva

運輸方式： 凍存運輸

器官來源： 其他

細胞形態(tài)： 成纖維樣

ATCC Number： CRL-1591?

是否是腫瘤細胞： 0

物種來源： 其他

數(shù)量： 大量

TRA-171細胞Designations: TRA-171

Depositors: G Kuno

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Toxorhynchites amboinensis

Morphology: fibroblast

Source: Organ: larva, whole

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. TRA-171細胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Virus Susceptibility: dengue virus types 1, 2, and 3; laboratory adapted dengue virus types 1, 2, 3, and 4

Age: larva

Propagation: ATCC complete growth medium: MThe base medium for this cell line is a 1:1 mixture of MM (Mitsuhashi and Maramorosch) and VP12 Medium (J. Med. Entomol. 6:432-439, 1969. PubMed: 5360492) To make the complete growth medium, add the following components to the base medium:

heat-inactivated fetal bovine serum to a final concentration of 10%.

Temperature: 28.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended

Medium Renewal: 2 to 3 times per week

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. TRA-171細胞Allow the flask to sit at room temperature until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: culture medium 95%; DMSO, 5%

Doubling Time: 28 hrs

References: 22662: Kuno G. New cell line. A continuous cell line of a nonhematophagous mosquito, Toxorhynchites amboinensis. In Vitro 16: 915-917, 1980.

26097: Varma MG, Pudney M. TRA-171細胞The growth and serial passage of cell lines from Aedes aegypti (L.) larvae in different media. J. Med. Entomol. 6: 432-439, 1969. PubMed: 5360492