Ltk-11細(xì)胞
生長狀態(tài)： 貼壁生長
細(xì)胞形態(tài)： 成纖維樣
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
品系： C3H/An
年限： 100 days *****
組織來源： subcutaneous connective tissue; areolar and adipose
數(shù)量： 大量
運(yùn)輸方式： 凍存運(yùn)輸
Ltk-11細(xì)胞ATCC Number： CRL-10422?
相關(guān)**： 正常
Designations: Ltk-11
Depositors: Synaptic Pharmaceutical Corporation
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus
Morphology: fibroblast
Source: Tissue: subcutaneous connective tissue; areolar and adipose
Strain: C3H/An
Disease: normal
Ltk-11細(xì)胞Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors: 5-hydroxytryptamine (serotonin) 1D beta (5HT1Dbeta)
Age: 100 days *****
Gender: male
Comments: This line is a derivative of L-M(TK-) (see ATCC CCL-1.3) that is stably transfected with the gene for the human 5HT1Dbeta receptor.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing:Ltk-11細(xì)胞 Protocol: Remove old medium, add fresh 0.25% trypsin, EDTA (0.02%) solution, rinse and remove. Allow cells to sit at room temperature (or at 37C) until cells detach (approximately 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Twice per week
Preservation: Freeze medium: Complete growth medium, 90%; DMSO, 10%
Storage temperature: liquid nitrogen vapor phase
Related Products: parental cell line:ATCC CCL-1.3
References: 90271: Ltk-11細(xì)胞Konkel M, et al. Compounds specific for the human .alpha.1d adrenergic receptor and uses thereof. U.S. Patent 6,706,716 dated Mar 16 2004
