TM1細(xì)胞

細(xì)胞形態(tài)： **樣

**類型： IgM

數(shù)量： 大量

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

運(yùn)輸方式： 凍存運(yùn)輸

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

ATCC Number： HB-169?

Designations: TM1

TM1細(xì)胞Depositors: F Grumet

Isotype: IgM

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against Leu-5; cytotoxic

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. TM1細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with human leukocytes (HLA phenotype A2,A28,Bw4,B27,B44,Cw2).

Spleen and lymph node cells were fused with Sp2/08A2 myeloma cells.

The antibody is cytotoxic for Leu-5 positive (Leu-5+) cells.

Tested and found negative for ectromelia virus (mousepox).

TM1細(xì)胞Propagation: ATCC complete growth medium: Modified RPMI 1640 medium (see fact sheet), 90%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 1173: Grumet FC, et al. TM1細(xì)胞A simplified new method for HLA-DR typing using the TM1 monoclonal antibody. Hum. Immunol. 6: 63-73, 1983. PubMed: 6601099