TK#1 [Embryonic stem cell line]細(xì)胞
運(yùn)輸方式： 凍存運(yùn)輸
生長狀態(tài)： 貼壁生長
數(shù)量： 大量
細(xì)胞類型： 胚胎干細(xì)胞
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
器官來源： 胚胎
年限： embryo
TK#1 [Embryonic stem cell line]細(xì)胞細(xì)胞形態(tài)： 上皮樣
ATCC Number： CRL-11383?
Designations: TK#1 [Embryonic stem cell line]
Depositors: TW Mak
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus deposited as mouse
Morphology: epithelial
Source: Organ: TK#1 [Embryonic stem cell line]細(xì)胞embryo
Cell Type: embryonic stem cell;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: The cell line was used to produce mutant mice with germ line transmission for the IRF-2 disruption.
Age: embryo
Comments: The construct pMIRF2neoB was introduced into D3 embryonic stem (ES) cells by electroporation to disrupt the IRF-2 locus.
The cell line generated is deficient for the interferon regulatory factor 2 (IRF-2) gene.
The cell line was used to produce mutant mice with germ line transmission for the IRF-2 disruption.
Heterozygous mice were inbred to obtain mice homozygous for the disrupted gene.
TK#1 [Embryonic stem cell line]細(xì)胞The line should be grown on feeder layers of irradiated (3000 rads) or mitomycin C treated (0.01 mg/ml for 90 minutes) primary mouse embryonic fibroblasts or STO cells (see ATCC CRL-1503 or ATCC 56-X, irradiated STO cells).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.1 mM 2-mercaptoethanol, 85%; fetal bovine serum, 15%
Temperature: 37.0°C
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
feeder layer cells:ATCC 56-X
References: 46928: TK#1 [Embryonic stem cell line]細(xì)胞Mak TW, Taniguchi T. Mouse lacking the expression of interferon regulatory factor 2 (IRF-2). US Patent 5,675,059 dated Oct 7 1997
