2A細(xì)胞

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

數(shù)量： 大量

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 人

ATCC Number： CRL-12013?

年限： fetus

器官來(lái)源： 腎臟

運(yùn)輸方式： 凍存運(yùn)輸

Designations: 2A

2A細(xì)胞Depositors: Chiron Viagene, Inc.

Biosafety Level: 2 [Cells contain adenoviral DNA sequences ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens deposited as human

Morphology:

Source: Organ: kidney

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Age: fetus

Comments: 2A細(xì)胞The 2A amphotropic retroviral packaging cell line was derived from the human embryonic kidney line, 293 (see ATCC CRL-1573).

293 cells were co-transfected with the methotrexate resistance vector, pFR400 and the MoMLV gag/pol expression vector, pSCV10. [57428]

After selection for transfected cells, individual drug resistant cell colonies were expanded, analyzed and selected for overexpression of MoMLV gag/pol. [57428]

293 (clone 2-3) was co-transfected with the phleomycin resistance vector, pUT507 and the amphotropic envelope expression vector, pMLPenvAmSph and subsequently cloned. [57428]

The 2A clone was selected for expression of relatively high levels of both gag/pol and amphotropic envelope proteins. [57428]

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:

O.1 mM Non-Essential Amino Acids (NEAA)

fetal bovine serum to a final concentration of 10%

2A細(xì)胞Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended

Medium Renewal: Every 2 to 3 days

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

parental cell line:ATCC CRL-1573

References: 43969: DePolo NJ, et al. 2A細(xì)胞The resistance of retroviral vectors produced from human cells to serum inactivation in vivo and in vitro is primate species dependent. J. Virol. 73: 6708-6714, 1999. PubMed: 10400768

57428: Barber JR, et al. Retroviral packaging cell lines. US Patent 5,591,624 dated Jan 7 1997