GAP 8.3細(xì)胞

ATCC Number： HB-12?

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

細(xì)胞形態(tài)： **樣

**類型： IgG2a; kappa light chain

生長狀態(tài)： 懸浮生長

Designations: GAP 8.3

GAP 8.3細(xì)胞Depositors: AE Berger

Isotype: IgG2a; kappa light chain

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against human leukocyte (monocytes, lymphocytes, granulocytes); against CD45

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Comments: Mice were immunized with MOLT-4 cells a human T cell line (MOLT-4, ATCC CRL-1582).

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

The antibody recognizes a 200000 dalton antigen analogous to the mouse T200 antigen and the rat common leukocyte antigen (it does not react with erythrocytes).

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

GAP 8.3細(xì)胞Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 914: Berger AE, et al. GAP 8.3細(xì)胞A human leukocyte antigen identified by a monoclonal antibody. Hum. Immunol. 3: 231-245, 1981. PubMed: 6975769