TK-1細(xì)胞
細(xì)胞形態(tài)： **樣
細(xì)胞類型： T**細(xì)胞
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
品系： AKR/Cum
生長狀態(tài)： 懸浮生長
數(shù)量： 大量
ATCC Number： CRL-2396?
運(yùn)輸方式： 凍存運(yùn)輸
相關(guān)**： **瘤
Designations: TK-1
Depositors: EC Butcher
TK-1細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus deposited as mouse
Morphology: lymphoblast
Source: Strain: AKR/Cum
Disease: lymphoma
Cell Type: T lymphocyte;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression: CD8 +; alpha4, beta7 integrin +
Comments: TK-1細(xì)胞TK1 is a T-cell lymphoma cell line originated by E. C. Butcher and I. L. Weissman from a spontaneous thymic lymphoma/leukemia that developed in an AKR/Cum mouse. [38634]
TK1 cells have a unique phenotype with high levels of the lymphocyte integrin, alpha 4, beta 7.
They bind constitutively to the murine mucosal vascular addressin, MAdCAM-1. [22636]
TK1 cells are useful for studies of alpha 4, beta 7 function and regulation. The cells are CD8 positive; they display approximately 1% of L-selectin of that expressed by normal lymph node cells.
Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.1 mM non-essential amino acids, 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 1 to 2 X 10(5) viable cells/ml. Do not allow the cell concentration to exceed 2 X 10(6) cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: TK-1細(xì)胞Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 22636: Berlin C, et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell 74: 185-195, 1993. PubMed: 7687523
38633: Berlin C, et al. alpha 4 integrins mediate lymphocyte attachment and rolling under physiologic flow. Cell 80: 413-422, 1995. PubMed: 7532110
38634: Butcher EC, et al. Organ specificity of lymphocyte migration: mediation by highly selective lymphocyte interaction with organ-specific determinants on high endothelial venules. Eur. J. Immunol. 10: 556-561, 1980. TK-1細(xì)胞PubMed: 6157544
