GAD-1細(xì)胞
ATCC Number： HB-184?
運(yùn)輸方式： 凍存運(yùn)輸
數(shù)量： 大量
生長狀態(tài)： 懸浮生長
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
細(xì)胞形態(tài)： **樣
**類型： IgG1
Designations: GAD-1
GAD-1細(xì)胞Depositors: DI Gottlieb
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against glutamic acid decarboxylase (GAD)
Permits/Forms: GAD-1細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Comments: Animals were immunized with GAD.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with GAD in cells from chickens, mice, rats, rabbits, cats and macaque monkeys.
The enzyme GAD is present in and is a marker for neurons that use gamma amino butyric acid as a neurotransmitter.
GAD-1細(xì)胞Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Related Products: redeposit of the following strain for patent purposes:ATCC HB-12470
References: 1196: Gottlieb DI, et al. GAD-1細(xì)胞Monoclonal antibodies to glutamic acid decarboxylase. Proc. Natl. Acad. Sci. USA 83: 8808-8812, 1986. PubMed: 2430303
