LP4.4細(xì)胞
數(shù)量： 大量
運(yùn)輸方式： 凍存運(yùn)輸
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 小鼠
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
ATCC Number： HB-232?
細(xì)胞形態(tài)： **樣
**類(lèi)型： IgG2b; kappa light chain
LP4.4細(xì)胞Designations: LP4.4
Depositors: H Kubagawa, JF Kearney
Isotype: IgG2b; kappa light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:LP4.4細(xì)胞 immunoglobulin; monoclonal antibody; against human IgM, lambda
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with a human IgM, kappa paraprotein (LP) which reacted with intermediate filaments.
Lymph node cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with most human IgM, lambda and some human IgM, kappa molecules.
It appears to react with a conformational determinant expressed preferentially on IgM, lambda complexes.
LP4.4細(xì)胞It also reacts with mu - surrogate light chain complexes, and provides a marker for pre-B and early B cell differentiation.
It does not react with isolated mu, lambda or kappa chains.
Propagation: ATCC complete growth medium: RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 22490: Ohno T, et al. LP4.4細(xì)胞A mouse monoclonal antibody reactive preferentially with human IgM lambda. Hybridoma 9: 473-480, 1990. PubMed: 1701760
