Lp1 MAB 2細(xì)胞
生長狀態(tài)： 懸浮生長
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
細(xì)胞形態(tài)： **樣
**類型： IgG2b
ATCC Number： CRL-1770?
運(yùn)輸方式： 凍存運(yùn)輸
數(shù)量： 大量
Designations: Lp1 MAB 2
Lp1 MAB 2細(xì)胞Depositors: RM McKinney
Isotype: IgG2b
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against Legionella pneumophila serogroup 1
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Lp1 MAB 2細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with the bacterial antigenic subgroup Knoxville 1 (ATCC 33153).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with a surface antigen on L. pneumophila and is useful in clinical diagnostic tests for subtyping L. pneumophila serogroup 1 isolates for epidemiologic studies using indirect immunofluorescence assays.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. Lp1 MAB 2細(xì)胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing: Medium Renewal: Every 2 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1190: Joly JR, et al. Development of a standard subgrouping scheme for Legionella pneumophila serogroup 1 using monoclonal antibodies. J. Clin. Microbiol. 23: 768-771, 1986. PubMed: 3517064
58069: McKinney RM, et al. Monoclonal antibodies to Legionella pneumophila serogroup 1: possible applications in diagnostic tests and epidemiologic studies. Zentralbl. Bakteriol. Mikrobiol. Hyg. I Abt. Lp1 MAB 2細(xì)胞Orig. A255: 91-95, 1983.
