THB-5 [HB-5, HB5]細(xì)胞

數(shù)量： 大量

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

運(yùn)輸方式： 凍存運(yùn)輸

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

細(xì)胞形態(tài)： **樣

**類型： IgG2a; kappa light chain

ATCC Number： HB-135?

Designations: THB-5 [HB-5, HB5]

Depositors: TF Tedder

Isotype: IgG2a; kappa light chain

THB-5 [HB-5, HB5]細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: THB-5 [HB-5, HB5]細(xì)胞immunoglobulin; monoclonal antibody; against human C3d receptor (CR2, Epstein-Barr virus (EBV) receptor); against CD21

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Comments: Animals were immunized with the SB human B cell line.

Spleen cells were fused with P3X63Ag8.653 myeloma cells.

The antibody reacts with the human C3d receptor (14500 dalton). This receptor also is the receptor for Epstein-Barr virus (EBV) on human B cells.

THB-5 [HB-5, HB5]細(xì)胞Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: RPMI 1640 medium, 85%; fetal bovine serum, 15%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 990: Weis JJ, et al. Identification of a 145,000 Mr membrane protein as the C3d receptor (CR2) of human B lymphocytes. Proc. Natl. Acad. Sci. USA 81: 881-885, 1984. THB-5 [HB-5, HB5]細(xì)胞PubMed: 6230668