G2b.2細(xì)胞

ATCC Number： HB-109?

數(shù)量： 大量

細(xì)胞形態(tài)： **樣

**類型： IgG2b

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

運(yùn)輸方式： 凍存運(yùn)輸

生長狀態(tài)： 懸浮生長

G2b.2細(xì)胞Designations: G2b.2

Depositors: P Parham

Isotype: IgG2b

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: G2b.2細(xì)胞hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against HLA DC1 (HLA DR)

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Comments: Animals were immunized with glycoprotein fraction from the Bristol 8 human B lymphoblastoid cell line.

Spleen cells were fused with NS-1 myeloma cells.

G2b.2細(xì)胞This line is a switch variant of Genox 3.53 (ATCC HB-103).

An IgG2a switch variant of Genox 3.53 also exists (G2a.5, ATCC HB-110).

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: RPMI 1640 medium with 0.1 mM hypoxanthine, 90%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 994: Parham P, et al. G2b.2細(xì)胞Isolation of heavy chain class switch variants of a monoclonal anti-DC1 hybridoma cell line: effective conversion of noncytotoxic IgG1 antibodies to antibodies. Hum. Immunol. 8: 141-151, 1983. PubMed: 6417068