LM2/1.6.11細(xì)胞

器官來源： 脾

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

ATCC Number： HB-204?

運(yùn)輸方式： 凍存運(yùn)輸

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

數(shù)量： 大量

細(xì)胞形態(tài)： **樣

**類型： mouse IgG1

Designations: LM2/1.6.11

LM2/1.6.11細(xì)胞Depositors: LJ Miller, TA Springer

Isotype: mouse IgG1

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Organ: spleen

Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against human Mac-1 antigen; against CD11b

Permits/Forms: LM2/1.6.11細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: Please acknowledge the origin of this cell line in all relevant publications by citing the following publication(s): [1617]

Comments: Animals were immunized with immunoprecipitates of human granulocyte lysates using TS1/18 (see ATCC HB-203) and boosted with live human granulocytes.

Spleen cells were fused with P3X63Ag8.653 myeloma cells.

The antibody reacts with the human Mac-1 antigen and is specific for the alpha chain.

The depositor states that the cell line should not be maintained in culture continuously for greater than four months or antibody production may be lost.

Cloning and freezing of the cells after minimal passage (with selection of positive clones) will prevent loss of antibody producing cells.

Tested and found negative for ectromelia virus (mousepox).

Propagation: LM2/1.6.11細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water and supplemented with 1.5 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml.

Subcultivation Ratio: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: recommended serum:ATCC 30-2020

Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X

References: 1617: Miller LJ, et al. LM2/1.6.11細(xì)胞Regulated expression of the Mac-1, LFA-1, p150,95 glycoprotein family during leukocyte differentiation. J. Immunol. 137: 2891-2900, 1986. PubMed: 2428876

32897: Zhang L, Plow EF. Overlapping, but not identical, sites are involved in the recognition of C3bi, neutrophil inhibitory factor, and adhesvie ligands by the alpha M beta 2 integrin. J. Biol. Chem. 271: 18211-18216, 1996. PubMed: 8663418