G-3-5細(xì)胞
ATCC Number： CRL-2252?
數(shù)量： 大量
生長狀態(tài)： 懸浮生長
運(yùn)輸方式： 凍存運(yùn)輸
細(xì)胞形態(tài)： **樣
**類型： mouse IgM
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
Designations: G-3-5
Depositors: SK Malhotra
G-3-5細(xì)胞Isotype: mouse IgM
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against alpha actinin
Permits/Forms: G-3-5細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions: The G-3-5 cell line (ATCC CRL-2252) and the J1-31 cell line (ATCC CRL-2253) are distributed for research purposes only. S. Malhotra releases the line subject to the following:1. The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of S. Malhotra.2. Any proposed commercial use of these cells must first be negotiated with S. Malhotra at 1058 Eilinita Avenue, Glendale, California 91208. Tel./FAX: (818) 236-2878.
Comments: Animals were immunized human brain homogenate of multiple sclerosis plaques obtained at autopsy.
Spleen cells were fused with NS-1 myeloma cells.
The antibody recognizes a novel intermediate filament associated protein (MW 100000) with similarity to alpha actinin.
This antibody intensely stains astroglia and related macroglia in cryostat sections of human and rat central nervous system tissue.
It is useful for immunofluorescence, immunocytochemistry, and Western blotting procedures.
Propagation: G-3-5細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml. Do not allow the cell concentration to exceed 1 X 10(6) cells/ml.
Interval: Maintain cultures at a cell concentration between 0.5 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 22389: Jeffcoat S, et al. G-3-5細(xì)胞A novel intermediate filament-associated protein: further characterization of the G.3.5 antigen. Cytobios 82: 81-99, 1995. PubMed: 7587376
22605: . . Ann. N.Y. Acad. Sci. 436: 492-494, 1984.
22973: Price KA, et al. Localization and characterization of an intermediate filament- associated protein. Cytobios 76: 157-173, 1993. PubMed: 8181306
