1D3細(xì)胞

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

ATCC Number： HB-305?

細(xì)胞形態(tài)： **樣

**類型： IgG2a; kappa light chain

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 褐鼠

1D3細(xì)胞Designations: 1D3

Depositors: DT Fearon, I Krop, MS Schlissel, AR de Fougerolles

Isotype: IgG2a; kappa light chain

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: 1D3細(xì)胞immunoglobulin; monoclonal antibody; against mouse CD19

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Comments: The hybridoma cell line 1D3 secretes a rat monoclonal antibody that reacts with the mouse pan-B cell marker CD19.

The line was produced by fusing NS-1 myeloma cells with spleen cells from Lewis rats that had been immunized with transfected K562 cells expressing the extracellular domain of murine CD19.

The antibody binds to mouse CD19 with high affinity, but does not react with human CD19.

It is useful flow cytometric analysis of total mouse B cells and for studies of signal transduction involving CD19.

Propagation: 1D3細(xì)胞ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate supplemented with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%

Temperature: 37.0°C

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Alternately, cultures can be established by centrifugation with subsequent resuspension in fresh culture medium.

Establish new cultures at 2 X 10 exp5 viable cells/ml and maintain at between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.

Preservation: Culture medium, 95%; DMSO, 5%

References: 34490: Krop I, et al. 1D3細(xì)胞Self-renewal of B-1 lymphocytes is dependent on CD19. Eur. J. Immunol. 26: 238-242, 1996. PubMed: 8566073