LL 97A (AlMy)細(xì)胞

器官來(lái)源： 肺

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞類型： 成纖維細(xì)胞

細(xì)胞形態(tài)： 成纖維樣

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 人

ATCC Number： CCL-191?

相關(guān)**： 其他**

年限： 48 years

數(shù)量： 大量

LL 97A (AlMy)細(xì)胞生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

Designations: LL 97A (AlMy)

Depositors: RG Crystal

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Organ: lung

Disease: idiopathic pulmonary fibrosis

Cell Type: LL 97A (AlMy)細(xì)胞fibroblast

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 12

D13S317: 11,13

D16S539: 11,13

D5S818: 13

D7S820: 9,11

THO1: 6,9.3

TPOX: 10,11

vWA: 14,17

Cytogenetic Analysis: diploid; relatively unstable

Isoenzymes: LL 97A (AlMy)細(xì)胞G6PD, B

Age: 48 years

Gender: male

Ethnicity: Caucasian

Propagation: ATCC complete growth medium: Ham's F12K medium, 85%; fetal bovine serum, 15%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Remove medium, rinse monolayer once with fresh 0.25% trypsin and let the culture sit for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks. LL 97A (AlMy)細(xì)胞Subculture every 6 to 8 days.