LL 86 (LeSa)細(xì)胞

器官來源： 肺

細(xì)胞形態(tài)： 成纖維樣

生長狀態(tài)： 貼壁生長

數(shù)量： 大量

細(xì)胞類型： 成纖維細(xì)胞

是否是腫瘤細(xì)胞： 0

物種來源： 人

年限： 18 years

ATCC Number： CCL-190?

LL 86 (LeSa)細(xì)胞相關(guān)**： 正常

運(yùn)輸方式： 凍存運(yùn)輸

Designations: LL 86 (LeSa)

Depositors: RG Crystal

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

LL 86 (LeSa)細(xì)胞Source: Organ: lung

Disease: normal

Cell Type: fibroblast

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Cytogenetic Analysis: normal human male; diploid

Isoenzymes: G6PD, B

Age: 18 years

LL 86 (LeSa)細(xì)胞Gender: male

Ethnicity: Caucasian

Comments: The line was derived from tissue normal tissue from a patient with osteogenic sarcoma.

Propagation: ATCC complete growth medium: Ham's F12K medium, 85%; fetal bovine serum, 15%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

LL 86 (LeSa)細(xì)胞Remove medium, rinse the monolayer once with fresh 0.25% trypsin and let the culture sit for 10 to 15 minutes at room temperature. Add fresh medium, aspirate and dispense into new flasks.