TE19細(xì)胞
生長狀態(tài)： 懸浮生長
細(xì)胞形態(tài)： **樣
**類型： IgM
運輸方式： 凍存運輸
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
ATCC Number： HB-211?
TE19細(xì)胞數(shù)量： 大量
Designations: TE19
Depositors: BF Haynes
Isotype: IgM
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: TE19細(xì)胞lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against human thymic epithelium (Hassall's bodies)
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Comments: Animals were immunized with human thymic stroma.
Spleen cells were fused with NS-1 myeloma cells.
TE19細(xì)胞The antibody reacts with a granular pattern of cells within the cellular portion of Hassall's bodies.
It defines an antigen that is acquired between 14 and 16 weeks of fetal gestation, and marks the terminal stages of epidermal keratinocyte maturation.
The antibody binds specifically to cells within the cellular portion of Hassall's bodies and also to stratum granulosum of skin epidermis on individuals with the A blood group antigen.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 80%; heat-inactivated fetal bovine serum, 20%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1637: Lobach DF, et al. TE19細(xì)胞The human thymic microenvironment. Phenotypic characterization of Hassall's bodies with the use of monoclonal antibodies. J. Immunol. 134: 250-257, 1985. PubMed: 2578044
