16-1-11N細胞
數(shù)量： 大量
是否是腫瘤細胞： 0
物種來源： 小鼠
ATCC Number： HB-16?
細胞形態(tài)： **樣
**類型： IgG2a; kappa light chain
生長狀態(tài)： 懸浮生長
運輸方式： 凍存運輸
Designations: 16-1-11N
16-1-11N細胞Depositors: DH Sachs, K Ozato
Isotype: IgG2a; kappa light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against H-2 K k; cytotoxic
Permits/Forms: 16-1-11N細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Mice were immunized with lymphocytes from C3H mice.
Spleen cells were fused with NS-1 myeloma cells.
The antibody cross-reacts with H-2p, H-2r and H-2Kq.
This line was obtained from the same fusion as ATCC HB-25 (16-3-1N) and demonstrates the same pattern of reactivity on a test panel as ATCC HB-25.
Tested and found negative for ectromelia virus (mousepox).
Propagation: 16-1-11N細胞ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
fetal bovine serum to a final concentration of 10%
1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 905: Ozato K, et al. 16-1-11N細胞Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens. J. Immunol. 124: 533-540, 1980. PubMed: 7188699
