AT3B-1細(xì)胞

器官來源： 前列腺

細(xì)胞形態(tài)： 上皮樣

是否是腫瘤細(xì)胞： 0

物種來源： 褐鼠

年限： 22 months

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

品系： Copenhagen

ATCC Number： CRL-2375?

AT3B-1細(xì)胞相關(guān)**： 其他**

Designations: AT3B-1

Depositors: KJ Pienta

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Organism: Rattus norvegicus deposited as rat

Morphology: epithelial

Source: Organ: prostate

Strain: Copenhagen

Disease: AT3B-1細(xì)胞malignant carcinoma

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Age: 22 months

Gender: male

Comments: AT3B-1 was derived from the AT-3 cell line. AT-3 was derived from a ***** malignant rat prostate tumor obtained from a 22 month old inbred Copenhagen rat by Isaacs et al. [39920]

The parental cells were grown in increasing concentrations of the drug doxorubicin until the final concentration of 0.001 mM was achieved.

These cells express a multidrug (MDR) resistant phenotype. They are more resistant to vinblastine compared to controls.

When P-glycoprotein was blocked, the AT3 B-1 cell line demonstrated drug efflux pump activity. Injection of AT3 B-1 cells into rats followed by doxorubicin treatment produced larger tumors compared to the parental controls. [39921]

AT3B-1細(xì)胞This cell line can be used to study chemotherapy resistance in prostate cancer.

Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.001 mM doxorubicin, 90%; fetal bovine serum, 10%

Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: Every 2 to 3 days

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: Culture medium, 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 39920: Isaacs JT, et al. AT3B-1細(xì)胞Establishment and characterization of seven Dunning rat prostatic cancer cell lines and their use in developing methods for predicting metastatic abilities of prostatic cancers. Prostate 9: 261-281, 1986. PubMed: 3774632

39921: Replogle-Schwab TS, et al. Development of doxorubicin resistant rat prostate cancer cell lines. Anticancer Res. 17: 4535-4538, 1997. PubMed: 9494564